Log in or Register to get access to full text downloads. Vaginal cytology pattern and birth features of female Wistar rats treated with graded doses of ethanolic extract of spondias mombin. Abstract Twenty-five virgin female wistar rats weighing between g and g and divided into five groups of five rats per group were treated with graded oral dosages of ethanolic extract of Spondias mombin.
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Microscopic evaluation of the types of cells present in vaginal smears has long been used to document the stages of the estrous cycle in laboratory rats and mice and as an index of the functional status of the hypothalamic-pituitary-ovarian axis. The estrous cycle is generally divided into the four stages of proestrus, estrus, metestrus, and diestrus. On cytological evaluation, these stages are defined by the absence, presence, or proportion of 4 basic cell types as well as by the cell density and arrangement of the cells on the slide.
The efficiency of producing timed pregnant or pseudopregnant mice can be increased by identifying those in proestrus or estrus. Visual observation of the vagina is the quickest method, requires no special equipment, and is best used when only proestrus or estrus stages need to be identified. Strain to strain differences, especially in coat color can make it difficult to determine the stage of the estrous cycle accurately by visual observation.
Skip to search form Skip to main content. The rodent estrous cycle: characterization of vaginal cytology and its utility in toxicological studies. Goldman and Ashley S.
One of our goals is to decipher the reproductive mysteries of pouched rat: sexual maturity, cycling, breeding, etc. This is a micro snapshot of what those slides looked liked when they they were collected and viewed fresh. We followed this protoco l and used these procedures to collect the cells and analyze the slides.
To study the estrogenic activity of the hydro-alcoholic extract of Bambusa arundinaceae leaves HEBA in female Wistar rats. HEBA were administered to ovariectomized rats for 7 days at three different doses viz. Estrogenic activity was evaluated by doing uterotropic assay, vaginal cytology and measurement of vaginal opening in female Wistar rats. Stark A, Madar Z.